A Study of the Antimony Trichloride Color Reaction for Vitamin A. Ii. the Dilution Curve of Cod Liver Oil with Antimony
نویسندگان
چکیده
In a previous paper of this series (1) we have shown that while the dilution curve for cod liver oil with antimony trichloride is not a linear function, a comparison can be made between the colorimetric assay of vitamin A in certain fish oils, with a chloroform solution of antimony trichloride, and the biological assay upon the same oils, when certain precautions are taken. In a second paper (2) it was shown that the color produced with the unsaponifiable substance from cod liver oil with the antimony trichloride reagent is a linear function of the amount of unsaponifiable substance used, below the maximum color which can be produced with a given concentration of antimony trichloride; that deviation of the dilution curve with oils from a linear function at relatively low color values is due to some interfering substance present in the oil (possibly due to the formation of a more stable compound between antimony trichloride and some substance or substances in the oil than is formed between antimony trichloride and the blue-producing substance of cod liver oil). In this paper dilution curves of several fish liver oils are given and a correlation shown between the calorimetric and biological assay of the oils.
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